The decaffeinated form of variety #1 coffee was similarly analyzed in triplicates (Figure 2) and also showed excellent reproducibility of analysis.
Two other varieties of coffee (variety #2 and #3) were analyzed, and the TIC overlay of the three coffee types (Figure 4)
showed some distinct profiles between the samples. For instance,
a peak at ~30 secs was common to #1 and #2, but not observed
in #3. The spectra of this peak was matched against the NIST
library and the probability of the match suggested it was likely
Literature studies suggest that methyl pyrrole degradation
is accelerated in coffee with high moisture content, which
may be the reason for variety #3 having little or no methyl
A brief study of coffee fingerprinting using the Torion T- 9
coupled to SPME sampling resulted in reproducible profiles for
triplicate analysis. Separation of the volatile/semi-volatile com-
pounds by GC and the specificity of detection provided by MS
along with spectral matching to the NIST library helped iden-
tify differences in coffee varieties. Therefore, portable GCMS
provides a rapid and reliable screening technology to study
coffee aroma profiles within a couple of minutes, making it an
ideal tool for on-site analysis.
Figure 3: Overlay of the TIC of caffeinated and decaffeinated coffee
variety #1 samples.
Figure 4: Overlay of the TICs of three different coffee varieties.
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